Denomination: Development of analytical methods for the determination of corticosteroid drug metabolites for treatment monitoring.

Reference: PTQ-17-09317

Start date: 01/12/2018

End Date: 11/30/2021

Call: State Subprogram for Incorporation and in the State Subprogram for Training, of the State Program for the Promotion of Talent and its Employability, within the framework of the State Plan for Scientific and Technical Research and Innovation 2017-2020 – Torres Quevedo contract and grant for the training of doctors in companies “Industrial Doctorates.


This Torres Quevedo project aims to develop and optimize sensitive and selective chromatographic methods (HPLC-DAD and HPLC-MS/MS) for the determination of glucocorticoids used in the treatment of asthmatic patients and their main metabolites, for application to urine samples. and plasma in routine clinical practice. In addition, the design and development of a rapid and simple analytical method is proposed, based on specific antibodies using the lateral flow immunochromatography (LFIA) technique and its application for monitoring adherence to the most widely used inhaled glucocorticoids.